Failure of aptamers to achieve required selectivity (Low, Med)
The fallback would be the use of commercial or custom-produced monoclonal antibodies. The selectivity of the aptamers will be established before the end of Year 1 to allow for implementation of this fallback. However, SELEX involves a counter SELEX in the selection process, so the risk of a lack of selectivity is very low.
The fallback would be the use of commercial or custom-produced monoclonal antibodies. The selectivity of the aptamers will be established before the end of Year 1 to allow for implementation of this fallback. However, SELEX involves a counter SELEX in the selection process, so the risk of a lack of selectivity is very low.
The fallback would be the use of commercial or custom-produced monoclonal antibodies. The selectivity of the aptamers will be established before the end of Year 1 to allow for implementation of this fallback. However, SELEX involves a counter SELEX in the selection process, so the risk of a lack of selectivity is very low.
Jesper Thestrup
1 February 2024
Barbara Luegger
Not yet
R2
Failure of aptamers to achieve required detection limits (Low, Med)
For most of the biomarkers the required detection limits are easily achievable. The challenging targets are hs-TnT and hs-CRP, and the proposal already has a built-in fallback by beginning the process of producing custom monoclonal antibodies from the beginning of the project. URV has observed that the use of hybrid antibody-aptamer sandwich assays improves detection limits as compared to either aptamer-aptamer or antibody-antibody combinations.
All project participants, and in particular the project managers and WP leaders, will be responsible for raising any material or perceived risk as part of the normal reporting process and to register all such risks and issues in the project’s online risk log.
